Difference between revisions of "Part:BBa K228861"

 
 
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<partinfo>BBa_K228861 short</partinfo>
 
<partinfo>BBa_K228861 short</partinfo>
  
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T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.  
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The OR Gate is made of a salicylate inducible promoter and a PO promoter, either salicylate or PhiR73 delta activator can activate the promoter and lead to the expression of the GFP gene.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 09:29, 15 October 2009

OR Gate-GFP

The OR Gate is made of a salicylate inducible promoter and a PO promoter, either salicylate or PhiR73 delta activator can activate the promoter and lead to the expression of the GFP gene. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1825