Difference between revisions of "Part:BBa K4283013:Design"

 
 
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Here we report an improved version of sulAp UV-inducible promoter, the double LexA Binding Unit RadiatioN Exercisable Regulator (dLexburner,). SulAp has been used for regulatory purposes and was initially designed and submitted by team UT-Tokyo in 2011 (iGEM11_UT-Tokyo, Part:BBa_K518010).
 
Here we report an improved version of sulAp UV-inducible promoter, the double LexA Binding Unit RadiatioN Exercisable Regulator (dLexburner,). SulAp has been used for regulatory purposes and was initially designed and submitted by team UT-Tokyo in 2011 (iGEM11_UT-Tokyo, Part:BBa_K518010).
  
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For detailed part infomation, please navigate: Part:BBa K4283013
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===Source===
 
===Source===
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===References===
 
===References===
 +
Lewis, L. K., Harlow, G. R., Gregg-Jolly, L. A., & Mount, D. W. (1994). Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. Journal of molecular biology, 241(4), 507-523.
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 +
Oertel-Buchheit, P., Porte, D., Schnarr, M., & Granger-Schnarr, M. (1992). Isolation and characterization of LexA mutant repressers with enhanced DNA binding affinity. Journal of molecular biology, 225(3), 609-620.
 +
 +
Little, J. W., & Gellert, M. (1983). The SOS regulatory system: control of its state by the level of RecA protease. Journal of molecular biology, 167(4), 791-808.
 +
 +
Sandler, S. J. (1994). Studies on the mechanism of reduction of W-inducible sulAp expression by recF overexpression in Escherichia coli K-12. Molecular and General Genetics MGG, 245(6), 741-749.

Latest revision as of 10:12, 13 October 2022


dLexburner SulAp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Here we report an improved version of sulAp UV-inducible promoter, the double LexA Binding Unit RadiatioN Exercisable Regulator (dLexburner,). SulAp has been used for regulatory purposes and was initially designed and submitted by team UT-Tokyo in 2011 (iGEM11_UT-Tokyo, Part:BBa_K518010).

For detailed part infomation, please navigate: Part:BBa K4283013

Source

biosynthesis

References

Lewis, L. K., Harlow, G. R., Gregg-Jolly, L. A., & Mount, D. W. (1994). Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. Journal of molecular biology, 241(4), 507-523.

Oertel-Buchheit, P., Porte, D., Schnarr, M., & Granger-Schnarr, M. (1992). Isolation and characterization of LexA mutant repressers with enhanced DNA binding affinity. Journal of molecular biology, 225(3), 609-620.

Little, J. W., & Gellert, M. (1983). The SOS regulatory system: control of its state by the level of RecA protease. Journal of molecular biology, 167(4), 791-808.

Sandler, S. J. (1994). Studies on the mechanism of reduction of W-inducible sulAp expression by recF overexpression in Escherichia coli K-12. Molecular and General Genetics MGG, 245(6), 741-749.