Difference between revisions of "Part:BBa K4279005"

 
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pUC57-SP lipase
 
pUC57-SP lipase
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== Profile ==
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Name: pUC57-SP lipase
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Base pairs: 1866bp
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== Experimental approach ==
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We obtained SP lipase gene through gene synthesis company. The target gene was constructed into pUC57 vector. The returned sequencing comparison results showed that we have successfully obtained this plasmid. Also, enzyme digestion showed the correct sequence.
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[[File:T-SubCat-China-BBa K4279005-figure 1.png|500px|thumb|center|Figure 1. Plasmid construct map of pUC57-SP lipase.]]
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[[File:T-SubCat-China-BBa K4279005-figure 2.png|500px|thumb|center|Figure 2. Enzyme digestion. Lane M: KB Ladder; Lane 1: pUC57-SP lipase plasmid; Lane 2: pUC57-SP lipase plasmid digested by EcoRI and BamHI.]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:59, 13 October 2022


pUC57-SP lipase

pUC57-SP lipase

Profile

Name: pUC57-SP lipase

Base pairs: 1866bp

Experimental approach

We obtained SP lipase gene through gene synthesis company. The target gene was constructed into pUC57 vector. The returned sequencing comparison results showed that we have successfully obtained this plasmid. Also, enzyme digestion showed the correct sequence.

Figure 1. Plasmid construct map of pUC57-SP lipase.
Figure 2. Enzyme digestion. Lane M: KB Ladder; Lane 1: pUC57-SP lipase plasmid; Lane 2: pUC57-SP lipase plasmid digested by EcoRI and BamHI.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2863
    Illegal NgoMIV site found at 2975
    Illegal NgoMIV site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1790
    Illegal SapI site found at 707