Difference between revisions of "Part:BBa K4444017:Design"
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===Source=== | ===Source=== | ||
| − | FKBP-nTEV: https://www.addgene.org/179675/ | + | FKBP-nTEV Backbone: https://www.addgene.org/179675/ |
mCherry: https://www.addgene.org/164037/ | mCherry: https://www.addgene.org/164037/ | ||
iLID: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID | iLID: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID | ||
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===References=== | ===References=== | ||
[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967 | [1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967 | ||
[2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y. | [2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y. | ||
Revision as of 04:20, 13 October 2022
Design Notes
Modifications to FKBP-nTEV:
- FKBP is replaced with iLID
- ER localization tag is added at beginning
- mCherry is added between iLID and nTEV
Components:
ER localization tag, iLID, mCherry, nTEV
Source
FKBP-nTEV Backbone: https://www.addgene.org/179675/
mCherry: https://www.addgene.org/164037/
iLID: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID
References
[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967 [2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.