Difference between revisions of "Part:BBa K4307027"

Revision as of 15:54, 12 October 2022

PmrC

This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 372
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

The following figure demonstrates our successful construction.

               Figure 1:  The construction results of PmrC.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4307027 short</partinfo>
-waiting
+This part is the promoter regulated by PmrB/A two-component system in <i>E.coli</i>. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 14: @@
 <partinfo>BBa_K4307027 SequenceAndFeatures</partinfo>
+<!-- Uncomment this to enable Functional Parameter display
+===Functional Parameters===
+<partinfo>BBa_K4307000 parameters</partinfo>
+<!-- -->
+<h2>Characterization </h2>
+<p>The following figure demonstrates our successful construction.</p>
+<br>
+https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307027-gel.png<br>
+                <b>Figure 1: </b> <b>The construction results of PmrC.</b>
 <!-- Uncomment this to enable Functional Parameter display