Difference between revisions of "Part:BBa K4390066"
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<partinfo>BBa_K4390066 short</partinfo> | <partinfo>BBa_K4390066 short</partinfo> | ||
| − | This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain. | + | This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain. |
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Revision as of 15:49, 12 October 2022
SpyCatcher O part
This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The glycine-serine linker forms an unstructured domain that allows for greater flexibility of the two SpyCatcher domains, while minimising the risk of the two domains hindering each other's functions. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]