Difference between revisions of "Part:BBa K4390035"

Revision as of 15:49, 12 October 2022

SpyTagged His-tagged M. edulis MT

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

SpyTag is a 13 amino acid-long protein that spontaneously reacts with the SpyCatcher protein (12.3 kDa). Together they form an intermolecular isopeptide bond. This provides an alternative to His-tag purification techniques. Insertion of either the SpyTag or the SpyCatcher sequence into a recombinant protein will allow its purification.

This construct is used to express SpyTagged Mytilus edulis Metallothionein, so that we can assemble a SpyTag-SpyCatcher hydrogel.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 212
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal PstI site found at 212
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 212
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 212
Illegal NgoMIV site found at 161
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4390035 short</partinfo>
-This construct is used to express SpyTagged Mytilus edulis Metallothionein, so that we can assemble a SpyTag-SpyCatcher hydrogel.
+'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+SpyTag is a 13 amino acid-long protein that spontaneously reacts with the SpyCatcher protein (12.3 kDa). Together they form an intermolecular isopeptide bond. This provides an alternative to His-tag purification techniques. Insertion of either the SpyTag or the SpyCatcher sequence into a recombinant protein will allow its purification.
+This construct is used to express SpyTagged Mytilus edulis Metallothionein, so that we can assemble a SpyTag-SpyCatcher hydrogel.
 <!-- -->