Difference between revisions of "Part:BBa K4307009"
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| + | Originated from <i>Lactococcus lactis subsp. Lactis</i>, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for <i>E.coli</i> strain.<br> | ||
| + | Functions together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307013) to express fluorescence signal induced by nisin. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K4307000 parameters</partinfo> | ||
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| + | <h2>Characterization</h2> | ||
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| + | <p>The following figure demonstrates our successful construction.</p> | ||
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| + | <br> | ||
| + | https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307009-gel.png<br> | ||
| + | <b>Figure 1: </b> <b>The construction results of PnisA.</b> | ||
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Revision as of 15:43, 12 October 2022
No part name specified with partinfo tag.
Originated from Lactococcus lactis subsp. Lactis, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for E.coli strain.
Functions together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307013) to express fluorescence signal induced by nisin.
Sequence and Features No part name specified with partinfo tag.
Characterization
The following figure demonstrates our successful construction.
Figure 1: The construction results of PnisA.