Difference between revisions of "Part:BBa K4390060"

 
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<partinfo>BBa_K4390060 short</partinfo>
 
<partinfo>BBa_K4390060 short</partinfo>
  
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
  
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<!-- Add more about the biology of this part here
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==Usage and Biology==
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===Usage and Biology===
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This is one of the three types of silica-binding tags we explored for immobilising metallothioneins to silica beads. L2NC-linker was also a silica-tag part used by the 2021 Edinburgh OG Team.
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The green-fluorescent recombinant protein was used as an indicator to show qualitatively and quantitatively whether the L2NC peptide has affinity towards silica beads. This was assayed by comparing the green fluorescence intensity between the initial lysate and the supernatant after addition of the silica beads. There should be a significant reduction in fluorescence intensity to prove that the L2NC peptide indeed binds to silica beads and thus, immobilises the sfGFP fused to it.
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390060 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390060 SequenceAndFeatures</partinfo>
  

Revision as of 15:41, 12 October 2022


L2NC-linker-tagged sfGFP

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

This is one of the three types of silica-binding tags we explored for immobilising metallothioneins to silica beads. L2NC-linker was also a silica-tag part used by the 2021 Edinburgh OG Team.

The green-fluorescent recombinant protein was used as an indicator to show qualitatively and quantitatively whether the L2NC peptide has affinity towards silica beads. This was assayed by comparing the green fluorescence intensity between the initial lysate and the supernatant after addition of the silica beads. There should be a significant reduction in fluorescence intensity to prove that the L2NC peptide indeed binds to silica beads and thus, immobilises the sfGFP fused to it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 467