Difference between revisions of "Part:BBa K4497030"
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<partinfo>BBa_K4497030 short</partinfo>
 
<partinfo>BBa_K4497030 short</partinfo>
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Components
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This part consists of the bidirectional Promoter Pbi-1 (BBa_K4497027), that is induced by the Transcription Factor tetracycline-inducable Transactivator (tTA). The promoter is made of Tetracycline repeat elements (TRE) with a minimal CMV promoter on both sides. The promoter induces expression of the downstream miRFP (BBa_K4497029), allowing for an miRFP signal (Ex661/Em680) readout on tTA induction of the promoter.
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[[File:MUC cycle.png|800px]]
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[[File:MUC cycle.png]]
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:40, 12 October 2022


tTA Induceable miRFP Reporter Components

This part consists of the bidirectional Promoter Pbi-1 (BBa_K4497027), that is induced by the Transcription Factor tetracycline-inducable Transactivator (tTA). The promoter is made of Tetracycline repeat elements (TRE) with a minimal CMV promoter on both sides. The promoter induces expression of the downstream miRFP (BBa_K4497029), allowing for an miRFP signal (Ex661/Em680) readout on tTA induction of the promoter.

MUC cycle.png



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal PstI site found at 919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal PstI site found at 919
    Illegal NotI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
    Illegal XhoI site found at 841
    Illegal XhoI site found at 847
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal PstI site found at 919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal PstI site found at 919
    Illegal NgoMIV site found at 622
    Illegal NgoMIV site found at 1104
  • 1000
    COMPATIBLE WITH RFC[1000]