Difference between revisions of "Part:BBa K4216030"

Revision as of 15:33, 12 October 2022

pOxyS Marionette

Input

DpB.005 is an Input device that can transcribe an electrical signal into the production of a protein that will act as a signal for our "processors" (BBa_K4216035/6/7). They are can be used to pass on signals to the Processors and downstream our signal processing pipeline. They can also be used as standalone for prototyping and debug of for specific applications

To know more about this part visit: https://2022.igem.wiki/paris-bettencourt/pOxyS

Electrogenetic TU used as a marionette (https://www.nature.com/articles/s41589-018-0168-3), pOxyS is supposed to be activated by a redox reaction itself drived by an electrode working with a potentiostat, if the device is working, it will activate the CDS of C71m(CinR)(=Bba_K4216020), CinR can then activate another promotor (Bba_K4216008).

Design

Here an overview of our design for BBa K4216030:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 972
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 772
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4216030 short</partinfo>
+===Input===
-Electrogenetic TU used as a marionette (https://www.nature.com/articles/s41589-018-0168-3), pOxyS is supposed to be activated by a redox reaction itself drived by an electrode working with a potentiostat, if the device is working, it will activate the CDS of C71m(CinR)(=Bba_K4216020), CinR can then activate another promotor (Bba_K4216008).
-===Usage and Biology===
 DpB.005 is an Input device that can transcribe an electrical signal into the production of a protein that will act as a signal for our "processors" (BBa_K4216035/6/7).
@@ Line 13: / Line 9: @@
 To know more about this part visit: https://2022.igem.wiki/paris-bettencourt/pOxyS
+Electrogenetic TU used as a marionette (https://www.nature.com/articles/s41589-018-0168-3), pOxyS is supposed to be activated by a redox reaction itself drived by an electrode working with a potentiostat, if the device is working, it will activate the CDS of C71m(CinR)(=Bba_K4216020), CinR can then activate another promotor (Bba_K4216008).
 ==Design==