Difference between revisions of "Part:BBa K4390064"

 
Line 6: Line 6:
 
sfGFP (BBa_I746916) with SpyTag fused to each end
 
sfGFP (BBa_I746916) with SpyTag fused to each end
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This is a type OC part, so it combines the 5' fusion site of a type O part with the 3' fusion site of a type C part. This was done so that this construct fits in the expression cassette with the rest of our modular proteins, as the TU for this protein part has fewer parts than the others.
 +
 +
The 5' fusion site starts with GCC encoding alanine so that the SpyTag starts in line, this works with the type N parts we have designed. Since it is the last protein part in the ORF, we end with a double stop and then the standard JUMP type C 3' fusion site
  
 
<!-- -->
 
<!-- -->

Revision as of 15:32, 12 October 2022


SpyTagged sfGFP

JUMP OC part sfGFP (BBa_I746916) with SpyTag fused to each end

Usage and Biology

This is a type OC part, so it combines the 5' fusion site of a type O part with the 3' fusion site of a type C part. This was done so that this construct fits in the expression cassette with the rest of our modular proteins, as the TU for this protein part has fewer parts than the others.

The 5' fusion site starts with GCC encoding alanine so that the SpyTag starts in line, this works with the type N parts we have designed. Since it is the last protein part in the ORF, we end with a double stop and then the standard JUMP type C 3' fusion site

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]