Difference between revisions of "Part:BBa K4237047"
 
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We crossed the constitutive promoters of the J23 family (J23119, J23100, J23104, J23113) in prokaryotes as well as plac with several artificially designed promoters (TE1, TE2, TE3, TE4, TE5) in yeast that have been recently reported in the literature. The bacterial constitutive promoter is loaded upstream of the yeast promoter to form a hybrid promoter. We tested these combinations in brewer's yeast as well as bacteria and found that these promoters stably drove gene expression in both yeast and bacteria, and showed a decreasing trend consistent with data reported in the literature. These data suggest that our design worked, and although these synthetic promoters may differ in intensity from when they work alone, at the functional level this can be left out of the discussion.
 
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Latest revision as of 15:28, 12 October 2022


J23119+TE1

consitutive promoter in bacteria and yeast


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


BNSC-China

We crossed the constitutive promoters of the J23 family (J23119, J23100, J23104, J23113) in prokaryotes as well as plac with several artificially designed promoters (TE1, TE2, TE3, TE4, TE5) in yeast that have been recently reported in the literature. The bacterial constitutive promoter is loaded upstream of the yeast promoter to form a hybrid promoter. We tested these combinations in brewer's yeast as well as bacteria and found that these promoters stably drove gene expression in both yeast and bacteria, and showed a decreasing trend consistent with data reported in the literature. These data suggest that our design worked, and although these synthetic promoters may differ in intensity from when they work alone, at the functional level this can be left out of the discussion.

Figure. 7 Characterization of PVan_CC and PVan_CC_THS.