Difference between revisions of "Part:BBa K3317006"
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===2022 BNSC-China improvement=== | ===2022 BNSC-China improvement=== | ||
| − | We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold. | + | We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy [BBa_K4237022] to increase the activation ploidy from approximately 20-fold to 34-fold. |
Latest revision as of 15:24, 12 October 2022
Inducible pVanCC promoter
Sensitive promoter against vanillinic acid and presents its maximum induction in the presence of 100mM RPU 3 of vanillinic acid with a leak of 2.4x10-3 RPU in absence of induction (Meyer, A. J et. al 2019) this part needs its regulatory protein (BBa_K3317004)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 18
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 42 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2022 BNSC-China improvement
We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy [BBa_K4237022] to increase the activation ploidy from approximately 20-fold to 34-fold.