Difference between revisions of "Part:BBa K4229078:Design"
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===Source=== | ===Source=== | ||
− | + | Is an improved version of BBa_K3009033, and was cloned out of it. | |
− | ===References== | + | ===References== |
+ | 1] S. Alberti, “Phase separation in biology,” 2017, doi: 10.1111/pbi.1280. | ||
+ | |||
+ | [2] M. C. Huber et al., “Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments,” Nat Mater, vol. 14, no. 1, pp. 125–132, Jan. 2015, doi: 10.1038/nmat4118. |
Latest revision as of 15:21, 12 October 2022
SPD5 with SnoopTag and SpyTag regulated by lamdapL promotor and LacI promotor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 443
Illegal EcoRI site found at 2862
Illegal EcoRI site found at 4036
Illegal SpeI site found at 1840 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 443
Illegal EcoRI site found at 2862
Illegal EcoRI site found at 4036
Illegal SpeI site found at 1840 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 443
Illegal EcoRI site found at 2862
Illegal EcoRI site found at 4036
Illegal BglII site found at 452
Illegal BglII site found at 2763 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 443
Illegal EcoRI site found at 2862
Illegal EcoRI site found at 4036
Illegal SpeI site found at 1840 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 443
Illegal EcoRI site found at 2862
Illegal EcoRI site found at 4036
Illegal SpeI site found at 1840
Illegal NgoMIV site found at 335 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
...
Source
Is an improved version of BBa_K3009033, and was cloned out of it.
=References
1] S. Alberti, “Phase separation in biology,” 2017, doi: 10.1111/pbi.1280.
[2] M. C. Huber et al., “Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments,” Nat Mater, vol. 14, no. 1, pp. 125–132, Jan. 2015, doi: 10.1038/nmat4118.