Latest revision as of 15:18, 12 October 2022

CuO-RBS- CA22-rrnB T1 Terminator in pCT5c

Description

The codon optimized carbonic anhydrase part (BBa_K4417021) was cloned into pCT5c plasmid (BBa_K4417000) using restriction digest.

Figure 1:Codon optimized CA in pCT5c plasmid.

NdeI and SacI sites were used to clone pCT5c plasmid (BBa_K4417000) with carbonic anhydrase gene ((BBa_K4417021)

Figure 2:CuO-RBS- CA22-rrnB T1 Terminator in pCT5c.

Due to time constraints, the cloning of this part into the plasmid has not been fully completed, but the design was feasible and rigorous.

Sequence and Features

Assembly Compatibility:

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4417022 short</partinfo>
-aa
+<h1>Description</h1>
+The codon optimized carbonic anhydrase part (BBa_K4417021) was cloned into pCT5c plasmid (BBa_K4417000) using restriction digest.
+[[File:Codon optimized CA in pCT5c plasmid.jpg|600px|thumb|center|'''Figure 1:'''Codon optimized CA in pCT5c plasmid.]]
-<!-- Add more about the biology of this part here
+<h1>Cloning Strategy</h1>
-===Usage and Biology===
+NdeI and SacI sites were used to clone pCT5c plasmid (BBa_K4417000) with carbonic anhydrase gene ((BBa_K4417021)
+[[File:CA22.jpg|600px|thumb|center|'''Figure 2:'''CuO-RBS- CA22-rrnB T1 Terminator in pCT5c.]]
+Due to time constraints, the cloning of this part into the plasmid has not been fully completed, but the design was feasible and rigorous.
 <!-- -->