Difference between revisions of "Part:BBa K4192140"
Jiangnanqi (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
A promoter consisting of PcysJ and PcysH in response to threonine concentration | A promoter consisting of PcysJ and PcysH in response to threonine concentration | ||
| − | Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of | + | Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of <i>E.coli</i> in response to extracellular threonine challenges. |
To enhance the strength of output signals, the promoters cysJp and cysHp were merged together to form a synthetic promoter cysJHp. | To enhance the strength of output signals, the promoters cysJp and cysHp were merged together to form a synthetic promoter cysJHp. | ||
| Line 12: | Line 12: | ||
[[File:Inducible_activity_of_PcysJ_and_PcysH.png|600px|thumb|center|]] | [[File:Inducible_activity_of_PcysJ_and_PcysH.png|600px|thumb|center|]] | ||
| − | <p style="text-align: center;"><b>Fig.1 Inducible activity of PcysJ, PcysH and PcysJH | + | <p style="text-align: center;"><b>Fig.1 Inducible activity of PcysJ, PcysH and PcysJH</b></p > |
Revision as of 15:17, 12 October 2022
PcysJH
A promoter consisting of PcysJ and PcysH in response to threonine concentration
Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of E.coli in response to extracellular threonine challenges.
To enhance the strength of output signals, the promoters cysJp and cysHp were merged together to form a synthetic promoter cysJHp.
cysJp and cysHp could be affected by threonine independently.
Fig.1 Inducible activity of PcysJ, PcysH and PcysJH
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]