Difference between revisions of "Part:BBa K4192143"
Jiangnanqi (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
PcysJH is regulator controlled by threonine concentration, which is combinations of PcysJ and PcysH. and its downstream gene expression activity increases with the increase of threonine concentration. | PcysJH is regulator controlled by threonine concentration, which is combinations of PcysJ and PcysH. and its downstream gene expression activity increases with the increase of threonine concentration. | ||
| − | MazF is a non-specific ribonuclease (toxin) in E. coli and MazE is an antitoxin that combines it. | + | MazF is a non-specific ribonuclease (toxin) in <i>E. coli</i> and MazE is an antitoxin that combines it. |
The system was used to construct a suicide switch in response to threonine concentration. When the concentration of threonine was high, MazF and MazE were co-expressed, and the two neutralized each other to ensure that the bacteria would not die. However, when the threonine concentration decreased, the expressions of MazE and MazF decreased simultaneously, but because the stability of the antitoxin MazE was lower than that of MazF, more free MazF in the bacteria would kill the bacteria. | The system was used to construct a suicide switch in response to threonine concentration. When the concentration of threonine was high, MazF and MazE were co-expressed, and the two neutralized each other to ensure that the bacteria would not die. However, when the threonine concentration decreased, the expressions of MazE and MazF decreased simultaneously, but because the stability of the antitoxin MazE was lower than that of MazF, more free MazF in the bacteria would kill the bacteria. | ||
Latest revision as of 15:15, 12 October 2022
PcysJ-PcysH-mazE-mazF
PcysJH is regulator controlled by threonine concentration, which is combinations of PcysJ and PcysH. and its downstream gene expression activity increases with the increase of threonine concentration.
MazF is a non-specific ribonuclease (toxin) in E. coli and MazE is an antitoxin that combines it.
The system was used to construct a suicide switch in response to threonine concentration. When the concentration of threonine was high, MazF and MazE were co-expressed, and the two neutralized each other to ensure that the bacteria would not die. However, when the threonine concentration decreased, the expressions of MazE and MazF decreased simultaneously, but because the stability of the antitoxin MazE was lower than that of MazF, more free MazF in the bacteria would kill the bacteria.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 482
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]