Difference between revisions of "Part:BBa K4294810"

Latest revision as of 15:13, 12 October 2022

TU pLux LuxR PFc, PFc+ R

Usage and Biology

It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.

Design

We use it in our Positive Feedback constitutive (BBa_K4294803) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4294810 short</partinfo>
-/
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.
+==Design==
+We use it in our Positive Feedback constitutive (BBa_K4294803) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805)
 <!-- -->