Difference between revisions of "Part:BBa K4195180"
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===Usage and Design=== | ===Usage and Design=== | ||
This part is used as the target of the detection system <partinfo>BBa_K4195187</partinfo>、<partinfo>BBa_K4195188</partinfo>、<partinfo>BBa_K4195189</partinfo> and <partinfo>BBa_K4195190</partinfo>. We build the circuit similar as <partinfo>BBa_K4195178</partinfo>. <br/> | This part is used as the target of the detection system <partinfo>BBa_K4195187</partinfo>、<partinfo>BBa_K4195188</partinfo>、<partinfo>BBa_K4195189</partinfo> and <partinfo>BBa_K4195190</partinfo>. We build the circuit similar as <partinfo>BBa_K4195178</partinfo>. <br/> | ||
| − | Reference | + | =====Reference===== |
| − | 1. J. M. S. Lazarte ''et al.'' | + | 1. J. M. S. Lazarte ''et al.'' Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)'' '''9''', (2021). |
Revision as of 15:08, 12 October 2022
Biology
This sequence is a conserved region of toxin gene pirB.[1]It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
Fig. 1 Schematic illustration of RENDR.
Usage and Design
This part is used as the target of the detection system BBa_K4195187、BBa_K4195188、BBa_K4195189 and BBa_K4195190. We build the circuit similar as BBa_K4195178.
Reference
1. J. M. S. Lazarte et al. Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel) 9, (2021).