Difference between revisions of "Part:BBa K4294811"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene. | ||
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| + | ==Design== | ||
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| + | We used this transcriptional unit in our Positive Feedback + Repressor (BBa_K4294804) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs. | ||
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Revision as of 15:07, 12 October 2022
TU constitutive LuxR for PFc, PFc+R
Usage and Biology
It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.
Design
We used this transcriptional unit in our Positive Feedback + Repressor (BBa_K4294804) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]