Difference between revisions of "Part:BBa K4497028"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4497028 short</partinfo> | <partinfo>BBa_K4497028 short</partinfo> | ||
| − | This part consists of the bidirectional Promoter Pbi-1, that is induced by the Transcription Factor tetracycline-inducable Transactivator (tTA). The promoter is made of Tetracycline repeat elements (TRE) with a minimal CMV promoter on both sides. | + | This part consists of the bidirectional Promoter Pbi-1 (BBa_K4497027), that is induced by the Transcription Factor tetracycline-inducable Transactivator (tTA). The promoter is made of Tetracycline repeat elements (TRE) with a minimal CMV promoter on both sides. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:06, 12 October 2022
tTA Induceable EYFP Reporter
This part consists of the bidirectional Promoter Pbi-1 (BBa_K4497027), that is induced by the Transcription Factor tetracycline-inducable Transactivator (tTA). The promoter is made of Tetracycline repeat elements (TRE) with a minimal CMV promoter on both sides.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 465
Illegal XbaI site found at 484
Illegal SpeI site found at 490
Illegal PstI site found at 471
Illegal PstI site found at 700 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 465
Illegal SpeI site found at 490
Illegal PstI site found at 471
Illegal PstI site found at 700
Illegal NotI site found at 476 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 465
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 465
Illegal XbaI site found at 484
Illegal SpeI site found at 490
Illegal PstI site found at 471
Illegal PstI site found at 700 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 465
Illegal XbaI site found at 484
Illegal SpeI site found at 490
Illegal PstI site found at 471
Illegal PstI site found at 700 - 1000COMPATIBLE WITH RFC[1000]