Difference between revisions of "Part:BBa K4294808"
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| − | + | ==Usage and Biology== | |
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| + | TetR forms a dimer and binds to tet operators in pTet promoter and blocks the assembly of the transcription machinery. pTet is a medium-strength promoter that is constitutive “ON” in the absence of TetR. It contains two tet operators, one inside the core promoter sequence and the other after the -10 hexamer, leading to efficient repression. The system is efficiently induced via anhydrotetracycline hydrochloride (aTc), a tetracycline derivative which binds Tet R with an ~35-fold higher binding constant, being an effective inducer at very low concentrations. Additionally, its antibiotic activity is ~100-fold lower and has a minimal toxic effect on E.coli cells in the concentration used for induction. | ||
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| + | ==Design== | ||
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| + | We used this transcriptional unit in our Open loop construct (BBa_K4294801) | ||
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Revision as of 15:01, 12 October 2022
TU constitutive LuxR for OL
Usage and Biology
TetR forms a dimer and binds to tet operators in pTet promoter and blocks the assembly of the transcription machinery. pTet is a medium-strength promoter that is constitutive “ON” in the absence of TetR. It contains two tet operators, one inside the core promoter sequence and the other after the -10 hexamer, leading to efficient repression. The system is efficiently induced via anhydrotetracycline hydrochloride (aTc), a tetracycline derivative which binds Tet R with an ~35-fold higher binding constant, being an effective inducer at very low concentrations. Additionally, its antibiotic activity is ~100-fold lower and has a minimal toxic effect on E.coli cells in the concentration used for induction.
Design
We used this transcriptional unit in our Open loop construct (BBa_K4294801)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]