Difference between revisions of "Part:BBa K4237022"
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We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold.  
 
We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold.  
  
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[[image: T--SCU-China--scu-2021-LACI.png|600px|center|]]
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                <img style="margin:20px auto 5px auto;" src="https://static.igem.wiki/teams/4237/wiki/results/fig11.png.png" width="60%">
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                <p style="color:Gray; padding:0px 30px 10px;">Figure. 6 Visualization of arithmetic LacI evaluation result. The triangle points out the desired variant strain #29. </p>
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Revision as of 14:56, 12 October 2022


PvanR_THS

Improve van induced promoter. RNA appratus is added downstream PvanCC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 90
    Illegal NheI site found at 237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
    Illegal BamHI site found at 104
    Illegal BamHI site found at 213
    Illegal BamHI site found at 251
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This a an improvement part of Part:BBa_K3317006

We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold.

Figure. 6 Visualization of arithmetic LacI evaluation result. The triangle points out the desired variant strain #29.