Difference between revisions of "Part:BBa K4289006"

Latest revision as of 14:50, 12 October 2022

pQE-30

pQE30-backbone is one of the E. coli expression vectors, which uses a T5 promoter to regulate the expression of exogenous genes that could be induced by IPTG. We inserted the hGK2 DNA fragment into the BamHI and SalI sites, and the 6×His tag is fused at the N-terminal of the protein and is used for protein purification. The vector is a low-copy-number plasmid. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony. This plasmid backbone can be used to express different proteins in the future.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4289006 short</partinfo>
-A plasmid backbone for the construction of recombinant plasmids.
+pQE30-backbone is one of the E. coli expression vectors, which uses a T5 promoter to regulate the expression of exogenous genes that could be induced by IPTG. We inserted the hGK2 DNA fragment into the BamHI and SalI sites, and the 6×His tag is fused at the N-terminal of the protein and is used for protein purification. The vector is a low-copy-number plasmid. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony. This plasmid backbone can be used to express different proteins in the future.
 <!-- Add more about the biology of this part here