Difference between revisions of "Part:BBa K4237022"
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<partinfo>BBa_K4237022 parameters</partinfo> | <partinfo>BBa_K4237022 parameters</partinfo> | ||
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| + | ===This a an improvement part of Part:BBa_K3317006=== | ||
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| + | We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold. | ||
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| + | [[image: T--SCU-China--scu-2021-LACI.png|600px|center|]] | ||
Revision as of 14:49, 12 October 2022
PvanR_THS
Improve van induced promoter. RNA appratus is added downstream PvanCC
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 90
Illegal NheI site found at 237 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 66
Illegal BamHI site found at 104
Illegal BamHI site found at 213
Illegal BamHI site found at 251 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This a an improvement part of Part:BBa_K3317006
We tested the response of the food additive vanillic acid response promoter PvanCC and found that its activation ploidy was low compared to the original literature, and to further increase its activation ploidy, we used the literature-reported, RNA-coordinated THS strategy to increase the activation ploidy from approximately 20-fold to 34-fold.
