Difference between revisions of "Part:BBa K4192110"

Latest revision as of 14:42, 12 October 2022

cdg-gacA

RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens. (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.

Characterization

This part has been connected to the downstream of Plac (BBa_R0010) and transferred into E. coli and Pseudomonas fluorescens 2P24 for testing.

The data are shown in the figure below:

Fig.1 Biofilm of Pseudomonas fluorescens 2P24

Through, Levene's test of equality of error vDariances, p<0.05, it is proved that the experimental results of different recombinant bacteria have significant differences.

Through Duncan’s multiple range test, there is little difference between wild type and cdg recombinant bacteria, and there was also little difference between cdg and gacA groups. The biofilm production of cdg+gacA recombinant bacteria is significantly higher than that of the other two groups. This shows that the co-expression effect of cdg and gacA genes is better than that of the two genes alone. The analysis shows that both cdg and gacA genes can increase the concentration of c-di-GMP in bacteria, co-expression can make the concentration of c-di-GMP exceed the threshold and regulate the production of a large number of biofilms.

We can draw a conclusion that increasing the copy number of cdg and gacA at the same time can significantly increase the production of biofilm. Related parallel experiments further verify our conclusion. However, the effect of separate expression of two genes still needs to be determined by further experiments.

To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
Illegal BamHI site found at 259
Illegal XhoI site found at 357
Illegal XhoI site found at 801
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 1289
Illegal AgeI site found at 759
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 210
Illegal BsaI.rc site found at 777

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4192110 short</partinfo>
-RBS-cdg-RBS-gacA is composed of <i>cdg</i> gene (BBa_K4192010), <i>gacA</i> gene (BBa_4192011) and strong RBS sequence of <i>Pseudomonas fluorescens</i>. (BBa_K4192000).
+RBS-<i>cdg</i>-RBS-<i>gacA</i> is composed of <i>cdg</i> gene (BBa_K4192010), <i>gacA</i> gene (BBa_4192011) and strong RBS sequence of <i>Pseudomonas fluorescens</i>. (BBa_K4192000).
 <i>cdg</i> and <i>gacA</i> encode regulatory factors DGC and GacA in <i>Pseudomonas fluorescens</i>, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.
@@ Line 12: / Line 12: @@
 <center><strong> Fig.1 Biofilm of <i>Pseudomonas fluorescens</i> 2P24 </strong></center>
 <p>Through, Levene's test of equality of error vDariances, p<0.05, it is proved that the experimental results of different recombinant bacteria have significant differences.</p>
-<p>Through Duncan’s multiple range test, there is little difference between wild type and cdg recombinant bacteria, and there was also little difference between cdg and gacA groups. The biofilm production of cdg+gacA recombinant bacteria iss significantly higher than that of the other two groups. This shows that the co-expression effect of cdg and gacA genes is better than that of the two genes alone. The analysis shows that both cdg and gacA genes can increase the concentration of c-di-GMP in bacteria, co-expression can make the concentration of c-di-GMP exceed the threshold, and regulate the production of a large number of biofilms.</p>
+<p>Through Duncan’s multiple range test, there is little difference between wild type and <i>cdg</i> recombinant bacteria, and there was also little difference between <i>cdg</i> and <i>gacA</i> groups. The biofilm production of <i>cdg+gacA</i> recombinant bacteria is significantly higher than that of the other two groups. This shows that the co-expression effect of <i>cdg</i> and <i>gacA</i> genes is better than that of the two genes alone. The analysis shows that both <i>cdg</i> and <i>gacA</i> genes can increase the concentration of c-di-GMP in bacteria, co-expression can make the concentration of c-di-GMP exceed the threshold and regulate the production of a large number of biofilms. </p>
-<p>We can draw a conclusion that increasing the copy number of cdg and gacA at the same time can significantly increase the production of biofilm. Related parallel experiments further verify our conclusion. However, the effect of separate expression of two genes still needs to be determined by further experiments.</p>
+<p>We can draw a conclusion that increasing the copy number of <i>cdg</i> and <i>gacA</i> at the same time can significantly increase the production of biofilm. Related parallel experiments further verify our conclusion. However, the effect of separate expression of two genes still needs to be determined by further experiments.</p>
 <p>To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114.</p>