Difference between revisions of "Part:BBa K4202034"

 
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The GFP gene is ligated to Pveg-2x  leader RNA-spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter Pveg-2x  leader RNA-spoVG RBS. The reporter gene GFP is connected to the downstream of the promoter.
 
The GFP gene is ligated to Pveg-2x  leader RNA-spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter Pveg-2x  leader RNA-spoVG RBS. The reporter gene GFP is connected to the downstream of the promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
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==Usage==
  
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<p>This is the measurement device of <partinfo>BBa_K4202025</partinfo>.</p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:37, 12 October 2022


Pveg-2x leader RNA-spoVG RBS-GFP for Bacillus subtilis

The GFP gene is ligated to Pveg-2x leader RNA-spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter Pveg-2x leader RNA-spoVG RBS. The reporter gene GFP is connected to the downstream of the promoter.

Usage and Biology

Usage

This is the measurement device of BBa_K4202025.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321