Difference between revisions of "Part:BBa K4202027:Experience"
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===Applications of BBa_K4202027=== | ===Applications of BBa_K4202027=== | ||
| − | + | ===Characterization=== | |
| + | <br> | ||
| + | <b>Vector construct</b> | ||
| + | <p>To characterization this part, we constructed the measurement device <partinfo>BBa_K4202035</partinfo> and then constructed it to the pHY300PLK plasmid. The vector was then transformed into <i>Bacillus subtilis</i> WB600 strain.</p> | ||
| + | <br> | ||
| + | <b>Characterization method</b> | ||
| + | <p>The transformant were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 0%, 0.1%, 0.5%, 1%, 2%, 5% sucrose (w/v) at the ratio of 1:100. After cultured in 37℃, 220rpm for 7 hours, the culture were diluted into PBS at the ratio of 1:1000 and were measured the fluorescence intensity through flow cytometry. The cluster of <i>Bacillus subtilis</i> should be easily gated from SSC-A vs. FSH-H figure. </p> | ||
| + | <br> | ||
| + | <div align="center">[[File:Gate.jpg|500px]]</div> | ||
| + | <div align="center"><b>Fig 1</b> The <i>Bacillus subtilis</i> in the SSC-A vs. FSH-H figure</div> | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K4202027 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K4202027 StartReviews</partinfo> | ||
Latest revision as of 14:32, 12 October 2022
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Applications of BBa_K4202027
Characterization
Vector construct
To characterization this part, we constructed the measurement device BBa_K4202035 and then constructed it to the pHY300PLK plasmid. The vector was then transformed into Bacillus subtilis WB600 strain.
Characterization method
The transformant were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 0%, 0.1%, 0.5%, 1%, 2%, 5% sucrose (w/v) at the ratio of 1:100. After cultured in 37℃, 220rpm for 7 hours, the culture were diluted into PBS at the ratio of 1:1000 and were measured the fluorescence intensity through flow cytometry. The cluster of Bacillus subtilis should be easily gated from SSC-A vs. FSH-H figure.
User Reviews
UNIQbc5c94f14573a5fc-partinfo-00000001-QINU UNIQbc5c94f14573a5fc-partinfo-00000002-QINU