Difference between revisions of "Part:BBa K4344009"
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Part of our Sequencing primer set used for verification of various cloning steps throughout our project. Part of primer sets for qPCR of EGFP copies together with following parts: BBa_K4344009 (EGFP-fwd-1), BBa_K4344010 (EGFP-fwd-2), BBa_K4344011 (EGFP-fwd-3), BBa_K4344012 (EGFP-rev-1), BBa_K4344013 (EGFP-rev-2), BBa_K4344014 (EGFP-rev-3).  
 
Part of our Sequencing primer set used for verification of various cloning steps throughout our project. Part of primer sets for qPCR of EGFP copies together with following parts: BBa_K4344009 (EGFP-fwd-1), BBa_K4344010 (EGFP-fwd-2), BBa_K4344011 (EGFP-fwd-3), BBa_K4344012 (EGFP-rev-1), BBa_K4344013 (EGFP-rev-2), BBa_K4344014 (EGFP-rev-3).  
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File: 6_cell_culture.png|thumbnail|left|500px|<b>Figure 6: Titration curves and primer efficiency values</b> All titration curves range from 10^10 to 10^2 copy per well. A: Titration curve of eGFP Primer Set 1. B: Titration curve of <i>UL19</i> Primer Set 1 C: Titration curve of eGFP Primer Set 2 D: Titration curve of <i>UL19</i> Primer Set 2 E: Titration curve of eGFP Primer Set 3  F: Titration curve of <i>UL19</i> Primer Set 3 G: Overview of calculated primer efficiency of eGFP Primer sets. H: Overview of calculated primer efficiency <i>UL19</i> Primer sets.]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4344009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4344009 SequenceAndFeatures</partinfo>

Revision as of 14:25, 12 October 2022


EGFP-fwd-1

Part of our Sequencing primer set used for verification of various cloning steps throughout our project. Part of primer sets for qPCR of EGFP copies together with following parts: BBa_K4344009 (EGFP-fwd-1), BBa_K4344010 (EGFP-fwd-2), BBa_K4344011 (EGFP-fwd-3), BBa_K4344012 (EGFP-rev-1), BBa_K4344013 (EGFP-rev-2), BBa_K4344014 (EGFP-rev-3).

Usage and Biology

Figure 6: Titration curves and primer efficiency values All titration curves range from 10^10 to 10^2 copy per well. A: Titration curve of eGFP Primer Set 1. B: Titration curve of UL19 Primer Set 1 C: Titration curve of eGFP Primer Set 2 D: Titration curve of UL19 Primer Set 2 E: Titration curve of eGFP Primer Set 3 F: Titration curve of UL19 Primer Set 3 G: Overview of calculated primer efficiency of eGFP Primer sets. H: Overview of calculated primer efficiency UL19 Primer sets.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]