Difference between revisions of "Part:BBa K4160017"
 
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<h2>Usage & biology</h2>
 
<h2>Usage & biology</h2>
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<a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a>, IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>). STAT-induced IL-10 was expressed in a pLS13 plasmid.</p><br>
+
<a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022,</a> IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>). STAT-induced IL-10 was expressed in a pLS13 plasmid.</p><br>
  
 
<h2>Characterization </h2>
 
<h2>Characterization </h2>

Latest revision as of 14:19, 12 October 2022


STAT-induced IL-10

This composite part encodes for STAT-induced IL-10 and is composed of the basic parts STAT promotor (BBa_K4160006) and IL-10 (BBa_K4160007).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 209
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 209
    Illegal BamHI site found at 227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage & biology

TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.1 Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (BBa_K4160008). STAT-induced IL-10 was expressed in a pLS13 plasmid.


Characterization

Characterization of STAT-induced IL-10 expression by the GEMS system can be found on the BBa_K4160008 page.


References

  1. Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z