Difference between revisions of "Part:BBa K4344017"
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<partinfo>BBa_K4344017 short</partinfo> | <partinfo>BBa_K4344017 short</partinfo> | ||
| − | + | Primer used in RT-qPCR for detection of GAPDH transcript together with part: BBa_K4344016 (GAPDH-fwd.) | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | We conducted RT-qPCR on our <i>UL19</i> siRNA treated samples in duplicates each. Obtained mean C<sub>q</sub> values for <i>UL19</i> transcript and GAPDH housekeeping gene transcript, ΔC<sub>q</sub>, ΔΔC<sub>q</sub> and the 2-Fold are listed in <b>Table 2</b>. 2-Fold expression is also visualized in <b>Figure 7</b>. For treatment with dsRNA and siRNA encapsulated in our vehicle, no change in expression can be detected. For treatment with siRNA delivered by a commercial transfection reagent, a reduction of nearly 50% is seen. | ||
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Latest revision as of 14:14, 12 October 2022
GAPDH-rev.
Primer used in RT-qPCR for detection of GAPDH transcript together with part: BBa_K4344016 (GAPDH-fwd.)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]