Difference between revisions of "Part:BBa K4390107"
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<partinfo>BBa_K4390107 short</partinfo> | <partinfo>BBa_K4390107 short</partinfo> | ||
| − | - | + | This part was used by Edinburgh-UHAS_Ghana team of 2022 to test cell-free transcription protocols. The construct contains an F30 flanked Squash aptamer (BBa_K4390096) under a strong T7 RNA promoter (BBa_I712074). When used in a cell-free transcription system, the construct is trancscribed by a T7 RNA polymerase constitutively, causing fluorescence when the RNA aptamer is in the presence of DFHBI or DFHO. |
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Latest revision as of 14:09, 12 October 2022
Constitutive Squash
This part was used by Edinburgh-UHAS_Ghana team of 2022 to test cell-free transcription protocols. The construct contains an F30 flanked Squash aptamer (BBa_K4390096) under a strong T7 RNA promoter (BBa_I712074). When used in a cell-free transcription system, the construct is trancscribed by a T7 RNA polymerase constitutively, causing fluorescence when the RNA aptamer is in the presence of DFHBI or DFHO.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]