Difference between revisions of "Part:BBa K4390110"
(→Usage and Biology) |
(→Reference) |
||
| Line 9: | Line 9: | ||
The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams ([[Part:BBa_K3946002|BBa_K3946002]]). | The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams ([[Part:BBa_K3946002|BBa_K3946002]]). | ||
| − | + | ||
| − | + | ||
<!-- --> | <!-- --> | ||
Latest revision as of 14:06, 12 October 2022
L2NC tag expression (control)
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
Usage and Biology
A truncated version of the L2 ribosomal protein from E. coli, for fusion to N-terminal of a protein using JUMP assembly (N-terminal 1-60 and C-terminal 203-273 amino acids of L2 [silica-binding regions of L2 only] 130 amino acids). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity, allowing the use of a smaller tag without compromising on binding affinity. From literature, the dissociation constant between L2NC silica tag and silica beads is 1.7nM (Kim et al., 2020). The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams (BBa_K3946002).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 108
- 1000COMPATIBLE WITH RFC[1000]
Reference
Kim S, Joo K, Jo B, Cha H. Stability-Controllable Self-Immobilization of Carbonic Anhydrase Fused with a Silica-Binding Tag onto Diatom Biosilica for Enzymatic CO2 Capture and Utilization. ACS Applied Materials & Interfaces. 2020;12(24):27055-27063.