Difference between revisions of "Part:BBa K4390109"
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==Usage and Biology== | ==Usage and Biology== | ||
Car9 is a short silica-binding tag to add to the N-terminal of a protein using JUMP assembly, including a short alanine-rich linker (AAAL). The tag facilitates immobilisation to silica surfaces with a dissociation constant (1 µM), enabling enzyme immobilisation or purification using silica-based spin columns. The advantage of using Car9 silica tag is its small size (1.87 kDa) would introduce smaller effect to the functional enzyme activity in theory (Coyle and Baneyx, 2014). | Car9 is a short silica-binding tag to add to the N-terminal of a protein using JUMP assembly, including a short alanine-rich linker (AAAL). The tag facilitates immobilisation to silica surfaces with a dissociation constant (1 µM), enabling enzyme immobilisation or purification using silica-based spin columns. The advantage of using Car9 silica tag is its small size (1.87 kDa) would introduce smaller effect to the functional enzyme activity in theory (Coyle and Baneyx, 2014). | ||
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+ | ==Reference== | ||
+ | Coyle B, Baneyx F. A cleavable silica-binding affinity tag for rapid and inexpensive protein purification. Biotechnology and Bioengineering. 2014;111(10):2019-2026. | ||
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Latest revision as of 14:05, 12 October 2022
Car9 tag expression (control)
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
Usage and Biology
Car9 is a short silica-binding tag to add to the N-terminal of a protein using JUMP assembly, including a short alanine-rich linker (AAAL). The tag facilitates immobilisation to silica surfaces with a dissociation constant (1 µM), enabling enzyme immobilisation or purification using silica-based spin columns. The advantage of using Car9 silica tag is its small size (1.87 kDa) would introduce smaller effect to the functional enzyme activity in theory (Coyle and Baneyx, 2014).
Reference
Coyle B, Baneyx F. A cleavable silica-binding affinity tag for rapid and inexpensive protein purification. Biotechnology and Bioengineering. 2014;111(10):2019-2026.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]