Difference between revisions of "Part:BBa K4317014"

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https://static.igem.org/mediawiki/parts/thumb/5/58/PtrnQ.png/800px-PtrnQ.png
 
https://static.igem.org/mediawiki/parts/thumb/5/58/PtrnQ.png/800px-PtrnQ.png
  
==Reference==
 
  
Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.
 
  
 
===Usage and Biology===
 
===Usage and Biology===
 
https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
 
https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
  
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==Reference==
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Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.
 
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==Sequence and Features==
 
==Sequence and Features==

Revision as of 13:59, 12 October 2022


PtrnQ

trnQ codes for arginine-specific tRNA from Basillus subtilis

800px-TrnQ_ref.jpeg.png

PtrnQ was only twice as high as that from the P43 promoter.

800px-PtrnQ.png


Usage and Biology

800px-Promoter_strength.png


Reference

Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]