Difference between revisions of "Part:BBa K4271010"

Revision as of 13:51, 12 October 2022

rpoD sigma factor

ropD gene (BBa_K4271010), comprises N-terminal 69 amino acid for polyP binding, E. coli RpoD (1-555 a.a), C-terminal 4.2 region for UreA promoter recognition (BBa_K4271011).

The rpoD sigma factor is a factor under the sigma-70 family and is a common sequence in E. coli. Activated by polyphosphate bondage, the sigma factor will be expressed and assist the RNA polymerase to bind to downstream ureA promoter (BBa_K4271011), thus expressing the mCherry (BBa_J18932) fluorescent protein for detection to determine engineering success. The usage of the rpoD sigma factor can be seen in BBa_K4271013 and BBa_K4271014.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 693
Illegal BglII site found at 741
Illegal BamHI site found at 1448
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1519

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+ropD gene (BBa_K4271010), comprises N-terminal 69 amino acid for polyP binding, E. coli RpoD (1-555 a.a), C-terminal 4.2 region for UreA promoter recognition (BBa_K4271011).
 The rpoD sigma factor is a factor under the sigma-70 family and is a common sequence in E. coli. Activated by polyphosphate bondage, the sigma factor will be expressed and assist the RNA polymerase to bind to downstream ureA promoter (BBa_K4271011), thus expressing the mCherry (BBa_J18932) fluorescent protein for detection to determine engineering success. The usage of the rpoD sigma factor can be seen in BBa_K4271013 and BBa_K4271014.