Difference between revisions of "Part:BBa K4260222"
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<partinfo>BBa_K4260222 short</partinfo> | <partinfo>BBa_K4260222 short</partinfo> | ||
| − | This part consists of T7 promoter + Lac Operator + RBS + RecA mini intein ESR1 protein with OmpA signal peptide, linker and AmilCP + T7 terminator. | + | This part consists of T7 promoter + Lac Operator + RBS + RecA mini intein ESR1 protein with OmpA signal peptide, linker and AmilCP + T7 terminator. It allows the expression of RecA mini intein ESR1 protein when IPTG is added to the used media. After induction with IPTG, several assays can be performed in order to measure EDCs through a change in coloration, using an AmilCP chromoprotein as N and C extein, and ligating after the ESR1 receptor protein binds to an EDC. |
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Latest revision as of 13:45, 12 October 2022
T7 promoter+LacO+RBS+RecA mini intein ESR1 protein with OmpA, linker and AmilCP+T7 terminator
This part consists of T7 promoter + Lac Operator + RBS + RecA mini intein ESR1 protein with OmpA signal peptide, linker and AmilCP + T7 terminator. It allows the expression of RecA mini intein ESR1 protein when IPTG is added to the used media. After induction with IPTG, several assays can be performed in order to measure EDCs through a change in coloration, using an AmilCP chromoprotein as N and C extein, and ligating after the ESR1 receptor protein binds to an EDC.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3146
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]