Difference between revisions of "Part:BBa K4291007"

Latest revision as of 13:35, 12 October 2022

gldh

Glutamate dehydrogenase (gldh) is a mitochondrial enzyme that is involved in the metabolism of glutamate to 2-oxoglutarate, and reversibly converts glutamate to α-ketoglutarate as part of the urea cycle. The gldh enzyme is found primarily in the liver, kidney, and cardiac muscle, while the liver has the highest concentration of gldh activity and lower levels in the brain, skeletal muscle, and leukocytes. GLDH has a housekeeping role in cell metabolism. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 403
Illegal XhoI site found at 1174
Illegal XhoI site found at 1216
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 589
Illegal AgeI site found at 1093
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 775
Illegal BsaI site found at 1024

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 gldh
-The tnaC regulatory gene from the tna operon of E. coli controls the transcription of its own operon through an attenuation mechanism relying on the accumulation of arrested ribosomes during inhibition of its own translation termination. In this project, the expression of tnaC was regulated by tnaCAB operon in the pTrc99K vector. This mechanism requires ribosomal arrest induced by the regulatory nascent TnaC peptide in response to free L-tryptophan (L-Trp).
+Glutamate dehydrogenase (gldh) is a mitochondrial enzyme that is involved in the metabolism of glutamate to 2-oxoglutarate, and reversibly converts glutamate to α-ketoglutarate as part of the urea cycle. The gldh enzyme is found primarily in the liver, kidney, and cardiac muscle, while the liver has the highest concentration of gldh activity and lower levels in the brain, skeletal muscle, and leukocytes. GLDH has a housekeeping role in cell metabolism.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===