Difference between revisions of "Part:BBa K4361111"
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Revision as of 13:35, 12 October 2022
Blc operator sequence
The blc operator sequence, comparable to Part:BBa_K4361100 but lacking some nucleotides at the 5' and 3' site. This sequence is made for the insertion into a plasmid to act as a repressor for the expression of downstream genes.
In BBa_K4361115 this part is used to create an expression vetor with the super folded GFP gene upfront the blc operator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage in Biology
BlcR is an allosteric transcription factor BlcR from the bacterium Agrobacterium tumefaciens. This plant bacterium is able to use GBL, a precursor of GHB, as an energy source. In the absence of GBL, GHB or SSA, BlcR will bind to the blc operator and acts as a repressor for the transcription of the blc proteins. When GBL, GHB or SSA binds to BlcR, it is released from the DNA and the blcA, blcB and blcC proteins are transcribed and digest GBL to succinate ( Figure 1 ).
