Difference between revisions of "Part:BBa K4238005"

Revision as of 13:34, 12 October 2022

C120-trucCYC1p+hbax+ADH1terminator
This Part functions as a blue light-sensitive kill switch when transformed into yeast with BBa_K4238003.
The C120-trucCYC1promoter causes dimeric EL222 to bind to the C120 sequence under blue light, allowing transcription of the downstream sequence. Therefore, hBax is expressed only when the yeast gets exposed to blue light, leading to yeast death. The apoptosis-inducible human Bax gene (BBa_K2365048) was used as the kill switch. hBax has been shown to be able to induce apoptosis in yeast. This kill switch is also expected to be utilized in the field of biosecurity, as apoptosis induces the thin cleavage of genome sequences. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 86
Illegal SpeI site found at 79
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 740
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
1000
COMPATIBLE WITH RFC[1000]

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 The apoptosis-inducible human Bax gene (<a href="https://parts.igem.org/Part:BBa_K2365048">BBa_K2365048</a>) was used as the kill switch. hBax has been shown to be able to induce apoptosis in yeast. This kill switch is also expected to be utilized in the field of biosecurity, as apoptosis induces the thin cleavage of genome sequences.
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 <!-- Add more about the biology of this part here
 ===Usage and Biology===