Difference between revisions of "Part:BBa K4238004"
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When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry. | When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:33, 12 October 2022
C120-trucCYC1p+mCherry+ADH1t
When transformed into yeast together with BBa_K4238003, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (BBa_K4238005) deriving from the human Bax gene is used instead of mCherry.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 86
Illegal SpeI site found at 79 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 1000COMPATIBLE WITH RFC[1000]