Difference between revisions of "Part:BBa K4202027"
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<partinfo>BBa_K4202027 short</partinfo> | <partinfo>BBa_K4202027 short</partinfo> | ||
| − | Three leader RNA is ligated to PsacB promoter and spoVG RBS downstream. Different sucrose concentrations can induce the expression of promoters.This device is used by ZJU-China 2022 | + | Three leader RNA is ligated to PsacB promoter and spoVG RBS downstream. Different sucrose concentrations can induce the expression of promoters.This device is planned to be used by ZJU-China 2022 for sucrose-controlled induction of expression of a large number of proteins in <i>Bacillus subtilis</i>. |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | ==Biology== | ||
| + | <p>Pveg is an endogenous strong promoter of <i>Bacillus subtilis</i>, and the leader RNA sequence is from PsacB, which is a endogenous sucrose-sensitive promoter of <i>Bacillus subtilis</i>.The leader RNA sequence contains a terminator overlaping with the binding site of sacY <b>[1] [2]</b>. When the sucrose existed, the sacY can bind to the bing site in the leader RNA and stablized the terminator, which enables the transcription. The absence of sucrose can lead to the formation of terminator structure, which will lead to the early termination of downstream genes.</p> | ||
| + | <p><b>Reference </b></p> | ||
| + | <p><b>[1]</b> Tortosa, P., & Le Coq, D. (1995). A ribonucleic antiterminator sequence (rat) and a distant palindrome are both involved in sucrose induction of the bacillus subtilis sacxy regulatory operon. Microbiology, 141(11), 2921–2927. https://doi.org/10.1099/13500872-141-11-2921</p> | ||
| + | <p><b>[2]</b> Clerte, C., Declerck, N., & Margeat, E. (2013). Competitive folding of anti-terminator/terminator hairpins monitored by Single Molecule Fret. Nucleic Acids Research, 41(4), 2632–2643. https://doi.org/10.1093/nar/gks1315</p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:32, 12 October 2022
Pveg-3X leader RNA for Bacillus subtilis
Three leader RNA is ligated to PsacB promoter and spoVG RBS downstream. Different sucrose concentrations can induce the expression of promoters.This device is planned to be used by ZJU-China 2022 for sucrose-controlled induction of expression of a large number of proteins in Bacillus subtilis.
Usage and Biology
Biology
Pveg is an endogenous strong promoter of Bacillus subtilis, and the leader RNA sequence is from PsacB, which is a endogenous sucrose-sensitive promoter of Bacillus subtilis.The leader RNA sequence contains a terminator overlaping with the binding site of sacY [1] [2]. When the sucrose existed, the sacY can bind to the bing site in the leader RNA and stablized the terminator, which enables the transcription. The absence of sucrose can lead to the formation of terminator structure, which will lead to the early termination of downstream genes.
Reference
[1] Tortosa, P., & Le Coq, D. (1995). A ribonucleic antiterminator sequence (rat) and a distant palindrome are both involved in sucrose induction of the bacillus subtilis sacxy regulatory operon. Microbiology, 141(11), 2921–2927. https://doi.org/10.1099/13500872-141-11-2921
[2] Clerte, C., Declerck, N., & Margeat, E. (2013). Competitive folding of anti-terminator/terminator hairpins monitored by Single Molecule Fret. Nucleic Acids Research, 41(4), 2632–2643. https://doi.org/10.1093/nar/gks1315
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]