Difference between revisions of "Part:BBa K4238007"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4238007 short</partinfo> | <partinfo>BBa_K4238007 short</partinfo> | ||
| − | + | This part was created to assay the recombination by the Cre-loxP system in yeast. | |
| + | |||
| + | When Cre is not present in the cell, mCherry (red fluorescent protein) flanked by loxP is expressed, while mNeonGreen (green fluorescent protein) in the back is not expressed because of the CYC1 terminator. On the other hand, if Cre is present in the cell, only mNeonGreen is expressed because the mCherry sequence is cut out. | ||
| + | Therefore, the recombination timescale and other parameters can be evaluated by observing the fluorescence color under a microscope. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:22, 12 October 2022
ADH1p+loxP+mCherry+CYC1t+loxP+mNeonGreen+ADH1t
This part was created to assay the recombination by the Cre-loxP system in yeast.
When Cre is not present in the cell, mCherry (red fluorescent protein) flanked by loxP is expressed, while mNeonGreen (green fluorescent protein) in the back is not expressed because of the CYC1 terminator. On the other hand, if Cre is present in the cell, only mNeonGreen is expressed because the mCherry sequence is cut out. Therefore, the recombination timescale and other parameters can be evaluated by observing the fluorescence color under a microscope.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]