Difference between revisions of "Part:BBa K4129125:Design"
 
Line 15: Line 15:
 
The individual parts were either salvaged from in-house plasmids or <i>de novo</i> synthesised:
 
The individual parts were either salvaged from in-house plasmids or <i>de novo</i> synthesised:
 
<li>BBa_K4129024 was salvaged from a in-house plasmid</li>
 
<li>BBa_K4129024 was salvaged from a in-house plasmid</li>
−
<li>FunsTF18 was <i>de novo</i> synthesised from IDT</li>
+
<li>FunsTF18 assembled from FunsTF02(<i>de novo</i> synthesised from IDT) and HbaR10(<i>de novo</i> synthesised from twist)</li>
 
<li>BBa_K4129115 was salvaged from a in-house plasmid</li>
 
<li>BBa_K4129115 was salvaged from a in-house plasmid</li>
 
<li>BBa_J06504 salvaged from BBa_K3046004 </li>
 
<li>BBa_J06504 salvaged from BBa_K3046004 </li>
 
<li>BBa_K3669007 was salvaged from a in-house plasmid</li>
 
<li>BBa_K3669007 was salvaged from a in-house plasmid</li>
 
<li>BBa_K678036 was salvaged from a in-house plasmid</li>
 
<li>BBa_K678036 was salvaged from a in-house plasmid</li>

Latest revision as of 13:18, 12 October 2022


The fungal synthetic expression system with FunsTF18 and 6xLexO-Pmin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 2935
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3122
    Illegal BamHI site found at 3461
    Illegal BamHI site found at 5317
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3610
    Illegal XhoI site found at 3987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 3027
    Illegal NgoMIV site found at 5953
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 5667
    Illegal SapI site found at 323


Design Notes

The assembly was standardised to assemble the cassettes in bulk.


Source

The individual parts were either salvaged from in-house plasmids or de novo synthesised:

  • BBa_K4129024 was salvaged from a in-house plasmid
  • FunsTF18 assembled from FunsTF02(de novo synthesised from IDT) and HbaR10(de novo synthesised from twist)
  • BBa_K4129115 was salvaged from a in-house plasmid
  • BBa_J06504 salvaged from BBa_K3046004
  • BBa_K3669007 was salvaged from a in-house plasmid
  • BBa_K678036 was salvaged from a in-house plasmid