Difference between revisions of "Part:BBa K4367014:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Will do later
+
The part has been designed to allow for the golden gate assembley MoClo, and as such contains MoClo overhangs for MoClo implementation. A 6xHis-tag is included for extraction of the protein.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Will do later
+
Abscisic acid (ABI) is derived from a plant hormone which regulates plant growth. cTEV is derived from the Tobacco Etch Virus protease.
  
 
===References===
 
===References===

Latest revision as of 13:17, 12 October 2022


ABI-cTEV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 952
    Illegal BamHI site found at 1034
    Illegal BamHI site found at 1363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 1370
    Illegal SapI.rc site found at 1270


Design Notes

The part has been designed to allow for the golden gate assembley MoClo, and as such contains MoClo overhangs for MoClo implementation. A 6xHis-tag is included for extraction of the protein.


Source

Abscisic acid (ABI) is derived from a plant hormone which regulates plant growth. cTEV is derived from the Tobacco Etch Virus protease.

References