Difference between revisions of "Part:BBa K4129123:Design"

 
 
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===Design Notes===
 
===Design Notes===
  
−
 
+
The assembly was standardised to assemble the cassettes in bulk.
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+
  
  
 
===Source===
 
===Source===
  
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This part was assembled using USER cloning of parts that were either amplied from in-house plasmids or synthesied as gene block.
+
The individual parts were either salvaged from in-house plasmids or <i>de novo</i> synthesised:
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+
<li>BBa_K4129024 was salvaged from a in-house plasmid</li>
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===References===
+
<li>FunsTF05 was <i>de novo</i> synthesised from IDT</li>
 +
<li>BBa_K4129115 was salvaged from a in-house plasmid</li>
 +
<li>BBa_J06504 salvaged from BBa_K3046004 </li>
 +
<li>BBa_K3669007 was salvaged from a in-house plasmid</li>
 +
<li>BBa_K678036 was salvaged from a in-house plasmid</li>

Latest revision as of 13:16, 12 October 2022


The fungal synthetic expression system with FunsTF05 and 6xLexO-Pmin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3258
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 5785
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3113
    Illegal XhoI site found at 3550
    Illegal XhoI site found at 4455
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 6421
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 6135
    Illegal SapI site found at 323


Design Notes

The assembly was standardised to assemble the cassettes in bulk.


Source

The individual parts were either salvaged from in-house plasmids or de novo synthesised:

  • BBa_K4129024 was salvaged from a in-house plasmid
  • FunsTF05 was de novo synthesised from IDT
  • BBa_K4129115 was salvaged from a in-house plasmid
  • BBa_J06504 salvaged from BBa_K3046004
  • BBa_K3669007 was salvaged from a in-house plasmid
  • BBa_K678036 was salvaged from a in-house plasmid