Difference between revisions of "Part:BBa K4361107"
 
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<partinfo>BBa_K4361107 short</partinfo>
 
<partinfo>BBa_K4361107 short</partinfo>
  
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This Part shows the linearized DNA sequence of the plasmid [[Part:pSB1C3]] after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes (see [[Part:BBa_K4361115]] and [[Part:BBa_K4361117]]).
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This Part shows the linearized DNA sequence of the plasmid [[Part:pSB1C3]] after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes. Gene can be inserted upfront a T7 promoter, this allows expression in presence of the T7 RNA polymerase. <i>CmR</i> gene ensures the resistance of chloramphenicol.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:16, 12 October 2022


pSB1C3, single letter prefix and suffix

This Part shows the linearized DNA sequence of the plasmid Part:pSB1C3 after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes. Gene can be inserted upfront a T7 promoter, this allows expression in presence of the T7 RNA polymerase. CmR gene ensures the resistance of chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.