Difference between revisions of "Part:BBa K4202045:Experience"
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<p>The transformant (<i>Bacillus subtilis</i> with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD<sub>600nm</sub>(L=1mm) reaches around 0.1, the OD<sub>600nm</sub>(L=1mm) were measured every 40minutes.</p> | <p>The transformant (<i>Bacillus subtilis</i> with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD<sub>600nm</sub>(L=1mm) reaches around 0.1, the OD<sub>600nm</sub>(L=1mm) were measured every 40minutes.</p> | ||
| − | ==Result== | + | ==<b>Result</b>== |
<p>Compared to the control group, the OD<sub>600nm</sub>(L=1mm) of strains containing the kill switch began to decrease after the <sub>600nm</sub>(L=1mm) reached 0.12. The early decrease of OD<sub>600nm</sub> indicated that the bacteria are dying, which means our kill switch is working.</p> | <p>Compared to the control group, the OD<sub>600nm</sub>(L=1mm) of strains containing the kill switch began to decrease after the <sub>600nm</sub>(L=1mm) reached 0.12. The early decrease of OD<sub>600nm</sub> indicated that the bacteria are dying, which means our kill switch is working.</p> | ||
<div align="center">[[File:Kill result.png|500px]]</div> | <div align="center">[[File:Kill result.png|500px]]</div> | ||
Latest revision as of 13:06, 12 October 2022
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how you used this part and how it worked out.
Method
Measurement device construction
The sequence of this part was synthesis by Genscript and constructed to the pHY300PLK by In-Fusion clone.
Characterization
The transformant (Bacillus subtilis with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD600nm(L=1mm) reaches around 0.1, the OD600nm(L=1mm) were measured every 40minutes.
Result
Compared to the control group, the OD600nm(L=1mm) of strains containing the kill switch began to decrease after the 600nm(L=1mm) reached 0.12. The early decrease of OD600nm indicated that the bacteria are dying, which means our kill switch is working.
Blue curve represents strains containing suicide switches and the yellow line represents strains containing the empty plasmid(CK)
User Reviews
UNIQf6c580a5ef14c9e4-partinfo-00000000-QINU UNIQf6c580a5ef14c9e4-partinfo-00000001-QINU