Difference between revisions of "Part:BBa K4238004"
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When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry. | When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:05, 12 October 2022
C120-trucCYC1p+mCherry+ADH1t</br>
When transformed into yeast together with BBa_K4238003, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (BBa_K4238005) deriving from the human Bax gene is used instead of mCherry.